Correlation between CYP26 expression and the level of endogenous RA in the tailbud. (A–D) Expression of CYP26 in wild-type embryos at E7.75, E8.25, and E9.0, respectively. Expression of the RARE-hsplacZ transgene in wild-type (E–H) and CYP26−/− (I–J) embryos. X-gal staining extends to the caudal end of the mutant embryos. (A) Anterior; (P) posterior; (n) node. The arrowhead (C,G,K) indicates a position of the caudal end and the asterisk (D,H,L) indicates the base of the allantois. Scale bar, 300 μm.