Fig. 6.

p653 protein and DNA interactions. (A) Coprecipitation experiments were performed with GST-p618 beads and 0.2 mg/ml of p653, using GST beads and BSA as controls. Supernatant (S) and pellet (P) fractions were analyzed by Western blotting. (B) DNA binding of p653 was investigated at 50°C for 25 min with 1.5 nM DNA (147 bp) and 0 to 2.5 μM p653. DNA without protein served as a negative control. Protein-bound and free DNAs were separated by electrophoresis on an 11% nondenaturing acrylamide gel and were visualized by autoradiography.