Fig. 7.

Assembly and protein-protein interactions of p800. (A) Temperature dependence of the assembly of p800 into filaments. p800 was purified by size exclusion chromatography, kept overnight at 4°C, and then incubated for 5 min at either 22°C or 50°C. The filaments were analyzed by electron microscopy after negative staining with uranyl acetate. (B and C) Interaction of p800 with p618 and p387. Coprecipitation experiments with GST-p618 (B) and GST-p387 (C) were performed with 0.3 mg/ml p800. GST beads and BSA were used as controls. Supernatants (S) and beads as the pellet fraction (P) were analyzed by Western blotting.