Fig. 1.

Rab6 traffics to the AC and colocalizes with pp150. (A) Rab6 expression in human foreskin fibroblasts (HFFs; uninfected and infected with HCMV). Infected cells were fixed at 5 days postinfection (dpi) and stained with rabbit anti-Rab6 antisera (green) and pp150 monoclonal antibody (MAb) (red). The anti-Rab6 antiserum is reactive with both isoforms of Rab6. (B, C) Expression of GFP-tagged Rab6A (B) and Rab9 (C) in uninfected or HCMV-infected HFFs. The cells were stained at 5 dpi with pp150 MAb (red). (D) Localization of GFP-Rab6A and endogenous BicD1 in transfected HFFs. HFFs were transfected with GFP-tagged Rab6A (green) and then stained with BicD1-specific rabbit antiserum (red). Images were collected by confocal microscopy (magnification, ×100). Nuclear dye TOTO3 was used to demarcate nuclei (blue).