Fig. 5.

Replication kinetics of HIV mutants. Stocks of WT and CA variant viruses on an NL4-3 HIV background were generated by transfection of constructs into HEK 293T cells and were normalized with respect to Gag levels. CEM-SS (A) or MT4 (B to D) cells were infected in parallel with normalized virus stocks of WT or variant viruses and passaged at 3- to 4-day intervals. Virus spread was monitored by measuring Gag levels in aliquots of infected cells by immunoblotting and densitometry. Replication is plotted as relative Gag level in infected cells versus number of days postinfection.