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Immunoblot of HCMV virions. Expression of gH, gL, pUL128, pUL130, and pUL131A was investigated in the parental TB40E virus and the four different TB40E-derived virus mutants encoding wild-type or mutant UL128. Viral stocks produced in HFF were lysed in reducing buffer and resolved by SDS-PAGE, and proteins were analyzed with MAb 17, directed against pUL128, or with rabbit antisera directed against gH, gL, pUL130, and pUL131A.
