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CD40 downregulation results from a loss of protein expression. (A) RAW264.7 cells were infected with the MCMV-GFP wild type or were left uninfected, and cytoplasmic RNA was extracted and analyzed for the presence of CD40 and actin transcripts. +, reverse transcriptase (RT) treated; −, without reverse transcriptase. RAW264.7 (B) and DC 2.4 (C) cells were mock infected or infected with MCMV-GFP, and CD40 (top) and β-actin (bottom) proteins were detected by immunoblotting.
