Figure 3.

Verification of microarray data by Northern blotting. (A) Northern blot analysis to verify target gene regulation using 10 μg of total RNA. Cells were harvested after 0 hr, 4 hr, and 8 hr of exposure to 300 nM 4-hydroxy tamoxifen (OHT). (B) Northern blot analysis to verify target gene regulation using 4 μg of poly A⁺ RNA. Cells were harvested after 0 hr, 4 hr, and 8 hr of exposure to 300 nM OHT.