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. 2001 Feb 1;15(3):267–285. doi: 10.1101/gad.864201

Figure 4.

Figure 4

Figure 4

E2F-induced changes in transcript levels are neither confined to transformed cells nor caused by overexpression of E2F fusion proteins. (A) Northern blot analysis of E2F-regulated genes in human diploid WI-38 ERE2F1 cells. 2 μg poly A RNA was loaded. (B) Northern blot analysis of RNA prepared from U2OS pRBΔcdk cells. 15 μg of total RNA was loaded. Hours indicate the time after removal of tetracyclin. Note the increase in the levels of the faster migrating mouse RbΔCDK with time. Blots were probed using a human probe, which likely is the reason that mouse RbΔcdk mRNA produced a much weaker signal than endogenous human RB1.