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Single-step growth of KOS and UL25 mutants. Vero or 8-1 cells were infected with wt KOS or UL25 mutant viruses at an MOI of 3 at 4°C for 1 h and incubated at 37°C to the indicated time point. Cells were lysed by multiple freeze-thaws, and viral progeny were quantified by plaque assay on 8-1 cells. The results shown are averages of duplicate experiments. (A) UL25 deletion mutants. (B) UL25 double and quadruple point mutants.
