Table 1.
Primers used for generation of recombinant HSV BACs
| BAC | UL25 Mutation | Primer sequencea (5′-3′) |
|---|---|---|
| bFH472 | Δ1-36 | tgtgttattttcgctctccgcctctcgcagacccccgagttcccccggTAGGGATAACAGGGTAATCGATTT |
| atccaaaagtcccgggggaactcgggggtctgcgagaggcggagagcGCCAGTGTTACAACCAATTAACC | ||
| bFH471 | Δ27-37 | gaacacaggcgcttcatagtcgccgattcccgaaacttctcgcccgtctttaacctccccTAGGGATAACAGGGTAATCGATTT |
| cgccgccgtctcccgggggaggttaaagacgggcgagaagtttcgggaatcggcGCCAGTGTTACAACCAATTAACC | ||
| bFH473 | Δ40-50 | gagttcccccgggacttttggatgtcgccccaggtggtcgtcctacagTAGGGATAACAGGGTAATCGATTT |
| tgtgcgctgggcctgtaggacgaccacctggggcgacatccaaaagtcGCCAGTGTTACAACCAATTAACC | ||
| bFH480 | F26A/I27A | aggcgcttcatagtcgccgattctagaaacgccgccaccTAGGGATAACAGGGTAATCGATTT |
| gtcccgggggaactcgggggtggcggcgtttctagaatcGCCAGTGTTACAACCAATTAACC | ||
| bFH481 | F35A/W36A | gaaacttcatcacccccgagttcccccgggacgcagcgatgtcgcccTAGGGATAACAGGGTAATCGATTT |
| aggttaaagacgggcgacatcgctgcgtcccgggggaactcgggggtgGCCAGTGTTACAACCAATTAACC | ||
| bFH482 | F26A/I27A/F35A/W36A | cttcatagtcgccgattcccggaatgccgccacccccgagttcccccgggaTAGGGATAACAGGGTAATCGATTT |
| aggttaaagacgggcgacatcgctgcgtcccgggggaactcgggggtgGCCAGTGTTACAACCAATTAACC | ||
| bFH471 | NTAP | cgaccgcagttctcgtgtgttattttcgctctccgcctctcgcagATGAAGCGACGATGGAAAAAG |
| gtgttcccagacgtccagagcgtcaaatgggcagtacgggtccatGCCCAGCTTGCAGCCGCCGGA |
a
Uppercase letters represent the template sequence (p-EP-Kan-S or p-EP-TAP-in), lowercase letters represent HSV UL25 gene sequences, underlined letters represent restriction sites, and letters in bold represent alanine substitution mutations.