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. 2011 May;85(10):5091–5104. doi: 10.1128/JVI.02565-10

Fig. 10.

Fig. 10.

Distribution of OuMV CP and MP in subcellular fractions during active viral infection or coexpressed through agroinfiltration in the absence of RNA1. (A) Western blot of proteins from virus infection (pG-CRNA1+2+3) and coagroinfiltration of RNA2 and RNA3 (pGC-RNA2+3) in the absence of RNA1. Samples were processed at 4 dpi. Arrows indicate the specific CP and MP bands. ab, antibody. (B) Control for proper separation between cytosolic and noncytosolic fraction. GFP was expressed from pBin-GFP through agroinfiltration, and samples for Western blotting were processed at 3 dpi. GFP-MP is a fusion of GFP with the movement protein obtained from agroinfiltration of pBin-GFP-MP and processed at 3 dpi. Sol, cytosolic fraction; P30, noncytosolic, membranous fraction; mock, pBin61-infiltrated leaf; ab, antibody. Bottom panels show Coomassie staining of a replicate of the gel used for Western blotting. Thermo Scientific Pierce Blue Prestained MW Marker (Pierce) is the molecular weight marker (MWM), and the sizes of the bands are expressed in thousands.