Fig. 2.

Schematic representation of constructs built and used in this study. Each clone has the same pJL89-derived backbone, where double StuI/SmaI restriction digestion allows insertion of blunt-end PCR fragments derived from RT-PCR of full-length genomic segments between the double 35S promoter (2-35S) and the hepatitis delta virus ribozyme (Rz) followed by a Nos terminator. Arrow directions indicate the orientation of the viral transcripts obtained for each of the described clones. The arrows indicate each ORF encoded by the genome.