Fig. 5.

Limited necrotic areas in upper uninoculated leaves when the plant is agroinfiltrated with a mix of RNA1- and RNA2-expressing clones. (A) Symptoms on upper uninoculated leaves at 12 days postinfiltration (dpi) with a combination of agrobacterium clones. The inset to the right shows an enlargement of the localized symptomatic area. (B) Western blotting for the presence of the OuMV MP (upper panel) and CP (middle panel). Samples were collected at 15 dpi in symptomatic (s) and asymptomatic (as) areas of an upper uninoculated leaf. Mock, inoculation with empty pBin61; virus, infection by mechanical inoculation of sap from N. benthamiana infected with OuMV VE9 isolate. The bottom panel shows Coomassie staining of the proteins loaded in the SDS-PAGE gel. Thermo Scientific Pierce Blue Prestained MW Marker (Pierce) is the molecular weight marker (MWM), and the sizes of the bands are expressed in thousands. The arrows indicate the position of the MP and CP bands in the Western blot. ab, antibody.