Fig. 8.

Subcellular localization of GFP-MP fusion in N. benthamiana protoplasts. (A, C, E, and G) Fluorescent signals of GFP (green) and chloroplasts (red). (B, D, F, and H) The same pictures overlaid with the corresponding bright-field images. Protoplasts transfected with the pGC-GFP-MP construct are shown in panels A to D. Besides the large aggregates visible in the cytoplasm (asterisks), GFP-MP fluorescence highlights numerous tubular protrusions of the protoplast plasma membrane. Such protrusions were never observed in protoplasts expressing the cytosolic GFP construct from the pGC-RNA3-GFP construct (E and F) or the endoplasmic reticulum-associated GFP-ER (G and H). n, nucleus. Bar, 20 μm.