Table 1.
Overview of test results for infectivity, necrotic local response, CP accumulation, and virion formation after inoculation using different combinations of agroclones and RNA transcripts
Inoculum type and RNA segment(s)a | Systemic infection | Necrotic local response | Virion formation in agroinflitrated leaves | CP accumulation in agroinfiltrated leaves |
---|---|---|---|---|
Agroclone(s) | ||||
RNA1(+) | − | − | − | − |
RNA2(+) | − | − | − | − |
RNA3(+) | − | − | − | + |
RNA1(+), RNA2(+), RNA3(+) | + | + | + | + |
RNA1(+), RNA2(+) | +b | + | − | − |
RNA1(+), RNA3(+) | − | − | + | + |
RNA3(+), RNA2(+) | − | − | − | + |
RNA1Δ3′, RNA2(+), RNA3(+) | − | − | + | + |
RNA transcript(s) | ||||
RNA1(+) | − | − | NAd | NA |
RNA2(+) | − | − | NA | NA |
RNA3(+) | − | − | NA | NA |
RNA1(+), RNA2(+), RNA3(+) | + | + | NA | NA |
RNA1(+), RNA2(+) | +c | + | NA | NA |
RNA1(+), RNA3(+) | − | − | NA | NA |
RNA3(+), RNA2(+) | − | − | NA | NA |
RNA1Δ3′, RNA2(+), RNA3(+) | − | − | NA | NA |
+, plus strand.
Infection was limited to an area of circa 0.5-cm diameter at some vascular tissue exit sites.
Rare infection foci at some vascular tissue exit sites.
NA, not applicable.