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. 2011 May;85(10):5081–5090. doi: 10.1128/JVI.02615-10

Fig. 8.

Fig. 8.

A cellular factor(s) sequence specifically binds the ZIIR element of Zp. (A) Autoradiogram of competition EMSAs showing sequence-specific binding to the ZIIR element. The location of the ZIIR-BP/DNA complex and free probe are indicated by arrows. (B) Autoradiogram of the UV cross-linking experiment to estimate the size(s) of ZIIR-BP. Lanes 1 through 3 are controls with no protein added, no UV cross-linking, and no endonuclease digestion after cross-linking. Sequences of the radiolabeled probe DNAs and unlabeled 2× ZIIRWT and 2× ZIIRmt competitor DNAs are provided in Materials and Methods.