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Viral DNA replication and gene expression is unaltered for the L172W MVMi mutant. (A) Southern blot of total DNA isolated from A9 cells after transfection with L172W and wild-type MVMi genomes, digestion with DpnI, and probing for MVM sequences. (B) Western blot analysis of protein isolated from A9 cells following transfection with L172W and wild-type MVMi genomes and probing with antibodies specific for the viral polypeptides NS1 or VP1 and VP2.
