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The L172W MVMi mutant produces only empty particles. (A) Iodixanol gradient of wild-type (upper panel) and L172W (lower panel) cell extracts analyzed for viral proteins by Western blotting. (B) Iodixanol gradient of wild-type (upper panel) and L172W (lower panel) cell extracts analyzed for viral genomes by alkaline agarose gel electrophoresis and probed for MVM DNA sequences.
