Fig. 6.

Transduction of TRIM22 in A3.01 T cells impairs HIV-1 LTR-driven luciferase expression. (A) TRIM22 (T22) expression in A3.01 cells was obtained by cell transduction with a TRIM22-expressing lentiviral vector (A3.01-TD T22), as shown by immunoblotting of WCE, followed by blotting with an anti-TRIM22 polyclonal Ab. Cells transduced with an empty vector (A3.01-TD CTRL), as well as the U937 permissive (P) and nonpermissive (NP) cells, are shown as a control. (B) Cell viability was measured by an MTT assay in transduced A3.01 cells. (C) TRIM22 inhibits HIV-1 transcription in TRIM22-expressing A3.01 cells infected with 16,000 cpm of minHIVLuc/well. The RLU were quantified at 48 h after infection. The results were obtained from a single experiment representative of three independently performed and are expressed as means ± the SEM (n = 3 replicas). The P value was calculated using an unpaired Student t test.