Table 2.
Impaired HIV-1 LTR-driven gene expression in nonpermissive cellsa
Virus | Mean log10 EC50 ± SEMb |
Pc | |
---|---|---|---|
Permissive | Nonpermissive | ||
minHIVGFP | −2.32 ± 0.04 | −2.32 ± 0.09 | 0.9750 |
HIVGFP | −2.13 ± 0.03 | −0.05 ± 0,19 | <0.0001 |
minHIVLuc | −2.72 ± 0.09 | −2.76 ± 0.11 | 0.8466 |
HIVLuc | −3.52 ± 0.09 | −2.69 ± 0.07 | <0.0001 |
The percentage of GFP+ cells or RLU was measured at 72 h after infection. Log10 EC50s were calculated by using a nonlinear regression curve fit of the Prism GraphPad software v.4.0.
The results are expressed as the mean log10 values of calculated the EC50s (n = 3).
P values were determined by use of the F-test comparing the best-fit EC50s.