Fig. 5.

Nuclear lamina disruption in MVM-infected cells is a transient event. LA9 cells were infected with MVM at an MOI of 4 and prepared for indirect IF at 2 or 21 h after infection. Cells were immunolabeled with antibodies against lamin A/C (green) and MVM (red). DNA was detected with DAPI (blue). Unlike at 2 h postinfection, no nuclear rim gaps are seen in the lamin A/C immunostaining at 21 h postinfection. Gaps in lamin A/C immunostaining are indicated by arrows. Scale bar, 5 μm.