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. 2011 May;85(10):5228–5231. doi: 10.1128/JVI.02562-10

Fig. 2.

Fig. 2.

The NS1 protein copurifies with NP but not the RNA polymerase. (A) TAP-tagged NP copurifies with NS1. 293T cells were transfected with plasmids expressing either TAP-tagged or untagged polymerase proteins or NP and NS1, as indicated. Cell lysates were harvested after 48 h, followed by TAP purification and Western blot analysis. (B) TAP-tagged NS1 copurifies with NP. 293T cells were transfected with plasmids expressing either TAP-tagged or untagged NS1 and various polymerase or NP proteins, as indicated, followed by TAP purification after 48 h and Western blot analysis. (C) NS1 does not copurify with the polymerase trimeric complex. 293T cells were transfected with plasmids expressing either TAP tagged or untagged proteins, as indicated, followed by TAP purification after 48 h and Western blot analysis.