Skip to main content
. 2001 Feb 1;15(3):316–327. doi: 10.1101/gad.855501

Figure 2.

Figure 2

Figure 2

Crescent is an efficient Wnt-8 antagonist. (A) Crescent injection into one cell of a two-cell embryo enlarged anterior structures and inhibited posterior extension in injected Xenopus embryos (bottom series of embryos). Control embryos (top) were injected with globin mRNA. LacZ mRNA was included as a lineage tracer. (B) Crescent inhibited the induction of siamois by chick Wnt-8c in Xenopus animal caps. RT–PCR analysis of siamois and ornithine decarboxylase (ODC) expression in whole embryos (WE; lane 1) or animal caps from embryos injected with the following RNAs: globin RNA (lane 2), 1 ng of crescent RNA (lane 3), 200 pg of chick Wnt-8c RNA (lane 4), 200 pg of chick Wnt-8c and 1 ng of crescent RNA (lane 5), 10 pg of mWnt-3a (lane 6), or 10 pg of mWnt-3a and 1 ng of crescent (lane 7). All injected RNA was made up to 1.2 ng with globin RNA. The difference between the levels of siamois expression in lanes 6 and 7 was approximately threefold, when normalized to ODC levels.