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. 2001 Feb 1;15(3):316–327. doi: 10.1101/gad.855501

Figure 4.

Figure 4

Wnt antagonists can induce cardiogenesis in PLP mesoderm but not in PPS explants. (A) Stage 5 posterior lateral plate (PLP) mesoderm (lanes 1,2) or posterior primitive streak (lanes 3,4) were infected with RCAS viruses encoding either alkaline phosphatase (AP; lanes 1,3) or crescent (lanes 2,4). Gene expression for the indicated genes was assayed by RT–PCR analysis. (B) Time course of Wnt-8c and Wnt-3a expression in stage 5 PLP and PPS mesoderm explants. PLP mesoderm (lanes 1–4) or PPS (lanes 5–8) were cultured for the indicated periods of time. At the end of the culture period, explants were harvested and transcript levels evaluated by RT–PCR. (C) Posterior tissues cocultured with COS cells expressing pCS2+–β-gal, pCMV–Dkk-1 (Xenopus), or pCS2+–crescent. PLP mesoderm (lanes 1,2,5,6) or PPS (lanes 3,4,7,8) were cultured with either control COS cells expressing CS2+–β-gal (lanes 1,3,5,7), COS cells expressing pCMV2–XDkk-1 (lanes 2,4), or COS cells expressing pCS2+-crescent (lanes 6,8). Transcript levels for the indicated genes were evaluated by RT–PCR.