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. 2001 Feb 1;15(3):316–327. doi: 10.1101/gad.855501

Figure 5.

Figure 5

Figure 5

Overexpression of Wnt genes blocks cardiogenesis in precardiac mesoderm. (A) Whole-mount in situ hybridization for Nkx-2.5 in chick embryos in which pellets of chick embryo dermal fibroblasts infected with either RCAS–mWnt-3a or control RCAS–AP were implanted into the precardiac region of embryos in New culture at stage 3+ to 4. Pellets of RCAS–Wnt-3a infected CEFs (red arrowheads) inhibit expression of Nkx-2.5 in a stage 9 embryo whereas pellets of control RCAS–AP-infected CEFs (open arrowheads) do not. (B) Red line in A indicates the level of this section. RCAS–Wnt3a-expressing cell pellets (red dotted circle) but not control cell pellets (black dotted circle) inhibit expression of cNkx-2.5 in the precardiac mesoderm and foregut endoderm but do not inhibit the accumulation of mesoderm lateral and ventral to the neural tube. (C) Ectopic Wnt expression suppresses cardiogenesis in anterior lateral plate mesoderm explants. Stage 5 anterior lateral plate mesoderm from the precardiac region was infected with either control virus (RCAS–GFP, lane 1; RCAS–AP, lane 3), or RCAS–Wnt-3a (lane 2) or RCAS–Wnt-8c (lane 4). Cultures were carried out in the presence of 200 ng/mL BMP-4 overnight followed by 48 h in 20 ng/mL BMP-4. Transcript levels were evaluated by RT–PCR.