Fig. 3.

Na induction of JNK is required for Na to activate lytic protein expression. (A) Hone-Akata cells were transfected with control vector or FLAG-Na expression plasmids, and immunoblot analysis was performed using antibodies against phosphorylated JNK (p-JNK), total JNK, FLAG (to detect transfected Na), and β-actin. (B) CNE2-Akata cells were transfected with control vector or FLAG-Na expression plasmids in the presence or absence of the JNK inhibitor SP600125 (10 μM). Cell lysates were harvested, and immunoblot analysis was performed to examine p-JNK, total JNK, BMRF1, Z, R, FLAG (Na), and β-actin expression levels.