Fig. 4.

Na interacts with TRAF2. 293 cells were cotransfected with the indicated combinations of vector control, FLAG-Na, or HA-TRAF2. Cell lysates were harvested in NP-40 buffer 48 h posttransfection. Immunoprecipitation analysis was performed using antibodies against the FLAG or HA tags of transfected FLAG-Na and HA-TRAF2. Immunoblot analysis was then performed using antibodies against FLAG or HA to detect the immunoprecipitated proteins. Direct load samples were also used for immunoblot analysis as a control.