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. 2011 May;85(9):4318–4329. doi: 10.1128/JVI.01856-10

Fig. 5.

Fig. 5.

Na and TRAF2 are localized to both the cytoplasm and nucleus. (A) Hone-Akata cells were transfected with YFP-tagged Na or TRAF2 expression plasmids. At 24 h posttransfection, the cells were fixed with paraformaldehyde and nuclei were stained with Hoechst 33342. Confocal fluorescence microscopy was used to visualize the YFP-tagged proteins and cell nuclei. (B). CNE2-Akata cells were transfected with vector control or FLAG-Na expression plasmids, in the presence or absence of cotransfected control siRNA or TRAF2-directed siRNA. At 48 h posttransfection, cell lysates were fractionated into nuclear and cytosolic fractions. Immunoblot analysis was performed using antibodies against TRAF2 and FLAG-Na. Tubulin and lamin A/C were included as controls for proper fractionation.