Fig. 7.

Na induces p100/p52 processing. CNE2-Akata cells were transfected with control or FLAG-Na expression plasmids and treated with TNF-α (10 ng/ml) for 20 or 60 min prior to harvesting. (A) Immunoblot analysis was performed using antibodies against IκBα, FLAG (Na), and β-actin. (B) Immunoblot analysis was performed using antibodies against p52, FLAG (Na), and β-actin. (C) AGS cells were transfected with control, LMP1, or FLAG-Na expression plasmids and harvested 48 h posttransfection. Biochemical fractionation was used to obtain nuclear (Nuc) and cytosolic (Cyto) extracts. Immunoblot analysis was performed to examine p100 processing by using a p52 antibody. Tubulin and lamin A/C antibodies were used as controls for fractionation.