Fig. 9.

Na and p53 synergistically induce lytic protein expression. Hone-Akata (A) or C666-1 (B) cells were cotransfected with control, FLAG-Na, or p53 expression plasmids; immunoblot analysis was performed 2 days later with antibodies against BMRF1 and Z to examine lytic gene expression and with antibodies against FLAG (Na) and p53 to examine levels of transfected Na and p53. (C) CNE2-Akata cells were cotransfected with combinations of control vector, FLAG-Na, and wild-type (wt) or mutant p53 (p53-175 and p53-248) expression vectors as indicated. Immunoblot analysis was performed 48 h posttransfection to examine BMRF1, FLAG (Na), and p53. β-Actin was used as an internal loading control.