Table 2.
Neutralization concentrations of MAbs 2909, PG16, and PG9 against 30 HIV-1 clade B Env pseudoviruses, either with wild-type Env or with the specific amino acid mutation(s) indicated
| Virus designationa | Residueb at position: |
IC50 (μg/ml) of: |
IC80 (μg/ml) of: |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 156 | 160 | 165 | 168 | 2909 | PG16 | PG9 | 2909 | PG16 | PG9 | |
| SF162 | N | K | I | K | 0.015 | >50 | >50 | 0.804 | >50 | >50 |
| SF162_K160N | N | N | I | K | >50 | 0.002 | 0.003 | >50 | 0.016 | 0.012 |
| AC10.29 | N | N | M | K | >50 | 0.009 | 0.012 | >50 | 0.038 | 0.073 |
| AC10.29_N160K | N | K | M | K | 0.001 | >50 | >50 | 0.004 | >50 | >50 |
| JR-FL | N | N | I | E | >50 | >50 | >50 | >50 | >50 | >50 |
| JR-FL_N160K | N | K | I | E | >50 | >50 | >50 | >50 | >50 | >50 |
| JR-FL_E168K | N | N | I | K | >50 | 0.003 | 0.008 | >50 | 0.015 | 0.055 |
| JR-FL_N160K_E168K | N | K | I | K | 0.001 | >50 | >50 | 0.005 | >50 | >50 |
| 7165.18 | N | N | I | K | >50 | 0.426 | >50 | >50 | >50 | >50 |
| 7165.18_N160K | N | K | I | K | 0.017 | >50 | >50 | 0.077 | >50 | >50 |
| WITO.33 | N | N | I | K | >50 | 0.002 | 0.005 | >50 | 0.006 | 0.009 |
| WITO.33_N160K | N | K | I | K | 0.105 | >50 | >50 | 0.411 | >50 | >50 |
| HT593.1 | N | N | I | K | >50 | 0.056 | 0.214 | >50 | 2.5 | 2.1 |
| HT593.1_N160K | N | K | I | K | 0.109 | >50 | >50 | 0.793 | >50 | >50 |
| YU2 | N | N | I | K | >50 | 0.258 | 3.86 | >50 | >50 | >50 |
| YU2_N160K | N | K | I | K | 0.492 | >50 | >50 | 2.3 | >50 | >50 |
| ADA | N | N | I | K | >50 | 0.012 | 0.128 | >50 | 0.060 | 5.2 |
| ADA_N160K | N | K | I | K | 1.24 | >50 | >50 | 50.0 | >50 | >50 |
| SC422.8 | N | N | I | K | >50 | >50 | 0.238 | >50 | >50 | >50 |
| SC422.8_N160K | N | K | I | K | 4.5 | >50 | >50 | >50 | >50 | >50 |
| TRO.11 | N | N | I | K | >50 | 0.077 | 29.4 | >50 | >50 | >50 |
| TRO.11_N160K | N | K | I | K | 5.0 | >50 | >50 | 50.0 | >50 | >50 |
| REJO.67 | N | N | P | K | >50 | 0.005 | 0.003 | >50 | 0.024 | 0.015 |
| REJO.67_N160K | N | K | P | K | 6.7 | >50 | >50 | >50 | >50 | >50 |
| JR-CSF | N | N | I | K | >50 | 0.001 | 0.002 | >50 | 0.008 | 0.008 |
| JR-CSF_N160K | N | K | I | K | >50 | >50 | >50 | >50 | >50 | >50 |
| 3988.25 | N | N | I | K | >50 | 0.005 | 0.016 | >50 | 0.022 | 0.062 |
| 3988.25_N160K | N | K | I | K | >50 | >50 | >50 | >50 | >50 | >50 |
| 5768.4 | N | N | L | K | >50 | 0.008 | 0.031 | >50 | 0.580 | 1.3 |
| 5768.4_N160K | N | K | L | K | >50 | >50 | >50 | >50 | >50 | >50 |
| SS1196.1 | K | N | I | K | >50 | 0.020 | 0.074 | >50 | 0.179 | 0.695 |
| SS1196.1_N160K | K | K | I | K | >50 | >50 | >50 | >50 | >50 | >50 |
| RHPA.7 | N | N | I | K | >50 | 0.334 | 10.0 | >50 | 3.5 | >50 |
| RHPA.7_N160K | N | K | I | K | >50 | >50 | >50 | >50 | >50 | >50 |
| THRO.18 | N | N | V | K | >50 | 0.498 | 13.2 | >50 | 50.0 | >50 |
| THRO.18_N160K | N | K | V | K | >50 | >50 | >50 | >50 | >50 | >50 |
| BaL.01 | N | N | I | K | >50 | 0.993 | 0.033 | >50 | >50 | 1.1 |
| BaL.01_N160K | N | K | I | K | >50 | >50 | >50 | >50 | >50 | >50 |
| TRJO.58 | N | N | T | K | >50 | 2.7 | 1.9 | >50 | >50 | 42.7 |
| TRJO.58_N160K | N | K | T | K | >50 | >50 | >50 | >50 | >50 | >50 |
| PVO.4 | N | N | I | R | >50 | 6.6 | 4.3 | >50 | >50 | 34.5 |
| PVO.4_N160K | N | K | I | R | >50 | >50 | >50 | >50 | >50 | >50 |
| CAAN.A2 | N | N | M | K | >50 | 25.0 | 14.4 | >50 | >50 | >50 |
| CAAN.A2_N160K | N | K | M | K | >50 | >50 | >50 | >50 | >50 | >50 |
| 6535.3 | N | N | R | K | >50 | >50 | 0.056 | >50 | >50 | 0.411 |
| 6535.3_N160K | N | K | R | K | >50 | >50 | >50 | >50 | >50 | >50 |
| 89.6 | N | Y | I | K | >50 | >50 | >50 | >50 | >50 | >50 |
| 89.6_Y160N | N | N | I | K | >50 | >50 | 0.140 | >50 | >50 | >50 |
| 89.6_Y160K | N | K | I | K | >50 | >50 | >50 | >50 | >50 | >50 |
| R2 | N | N | I | K | >50 | >50 | >50 | >50 | >50 | >50 |
| R2_N160K | N | K | I | K | >50 | >50 | >50 | >50 | >50 | >50 |
| R2_A162T | N | N | I | K | >50 | >50 | 10.3 | >50 | >50 | >50 |
| R2_N160K_A162T | N | K | I | K | >50 | >50 | >50 | >50 | >50 | >50 |
| HXB2 | N | N | I | K | >50 | >50 | >50 | >50 | >50 | >50 |
| HXB2_N160K | N | K | I | K | >50 | >50 | >50 | >50 | >50 | >50 |
| 6101.10 | N | D | I | K | >50 | >50 | >50 | >50 | >50 | >50 |
| 6101.10_D160N | N | N | I | K | >50 | >50 | >50 | >50 | >50 | >50 |
| 6101.10_D160K | N | K | I | K | >50 | >50 | >50 | >50 | >50 | >50 |
| QH0692.42 | N | S | I | K | >50 | >50 | >50 | >50 | >50 | >50 |
| QH0692.42_S160N_P162T | N | N | I | K | >50 | >50 | >50 | >50 | >50 | >50 |
| QH0692.42_S160K_P162T | N | K | I | K | >50 | >50 | >50 | >50 | >50 | >50 |
| BL01 | N | K | L | K | >50 | >50 | >50 | >50 | >50 | >50 |
| BL01_K160N | N | N | L | K | >50 | >50 | >50 | >50 | >50 | >50 |
| BR07 | N | H | R | K | >50 | >50 | >50 | >50 | >50 | >50 |
| BR07_H160N | N | N | R | K | >50 | >50 | >50 | >50 | >50 | >50 |
| BR07_H160K | N | K | R | K | >50 | >50 | >50 | >50 | >50 | >50 |
| QH0515.1 | N | N | I | R | >50 | >50 | >50 | >50 | >50 | >50 |
| QH0515.1_N160K | N | K | I | R | >50 | >50 | >50 | >50 | >50 | >50 |
| Neutralization breadthc for: | ||||||||||
| Wild-type viruses (n = 30) | 1 (3%) | 18 (60%) | 20 (67%) | 1 (3%) | 11 (37%) | 13 (43%) | ||||
| Mutant viruses (n = 30) | 10 (33%) | 2 (7%) | 3 (10%) | 8 (27%) | 2 (7%) | 2 (7%) | ||||
a
The viruses are ordered first by the 2909 IC50, then by the PG16 IC50, and finally by the PG9 IC50.
b
Residues at positions 156, 160, 165, and 168 (based on HXB2 numbering) with potential importance in the PG and 2909 epitope are shown.
c
Neutralization breadth was calculated as the number (percentage) of viruses neutralized with an antibody IC50 or IC80 of ≤50 μg/ml.