Table 1.
Epitope tag insertion mutants of PCVs
| Strain ID | Parent strain | Insert location | Insert tag | Insert size (aa) | Primersa | Titer (TCID50/ml) |
|---|---|---|---|---|---|---|
| PCV2b | NAb | NA | NA | NA | 1 + 2 | 104.5 |
| PCV1-2 | NA | NA | NA | NA | 11 + 12 | 103.7 |
| 2-ORF1N-HA | PCV2 | N′-ORF1 | HA | 9 | 1 + 3, 2 + 4 | |
| 2-ORF1C-HA | PCV2 | C′-ORF1 | HA | 9 | 1 + 5, 2 + 6 | |
| 2-ORF2N-HA | PCV2 | N′-ORF2 | HA | 9 | 1 + 7, 2 + 8 | 103.5 |
| 2-ORF2C-HA | PCV2 | C′-ORF2 | HA | 9 | 1 + 9, 2 + 10 | 105.0 |
| PCV1-2-HA1 | PCV1-2 | C′-ORF2 | 1× HA | 9 | 12 + 13, 11 + 14 | 103.7 |
| PCV1-2-HA2 | PCV1-2 | C′-ORF2 | 2× HA | 18 | 12 + 15, 11 + 16 | 103.7 |
| PCV1-2-HA3 | PCV1-2 | C′-ORF2 | 3× HA | 27 | 12 + 17, 11 + 18 | 103.5 |
| PCV1-2-GLU | PCV1-2 | C′-ORF2 | GLU | 10 | 12 + 19, 11 + 20 | 103.5 |
| PCV1-2-KT3 | PCV1-2 | C′-ORF2 | KT3 | 11 | 12 + 21, 11 + 22 | 103.7 |
Primer sequences (5 prime to 3 prime): primer 1, AGCCCGCGGAAATTTCTGACAAACGTTAC; primer 2, TTTCCGCGGGCTGGCTGAACTTTTGAAAG; primer 3, ACATGTACCCATACGATGTTCCAGATTACGCTCCCAGCAAGAAGA; primer 4, GGAGCGTAATCTGGAACATCGTATGGGTACATGTTGCTGCTGAGG; primer 5, ACTACCCATACGATGTTCCAGATTACGCTTGAGTCTTTTTTATCAC; primer 6, ACTCAAGCGTAATCTGGAACATCGTATGGGTAGTAATTTATTTCA; primer 7, GTAGCGTAATCTGGAACATCGTATGGGTACATAGCTGAAAACGAAA; primer 8, CTATGTACCCATACGATGTTCCAGATTACGCTACGTATCCAAGGAG; primer 9, CATTAAGCGTAATCTGGAACATCGTATGGGTAAGGGTTAAGTGGGG; primer 10, CTTACCCATACGATGTTCCAGATTACGCTTAATGAATAATAAAAAC; primer 11, GGAGGTACCCGAAGGCCGATTTGAAGCAG; primer 12, TCGGGTACCTCCGTGGATTGTTCTCCAGC; primer 13, ATTTAAGCGTAATCTGGAACATCGTATGGGTAGGGTTTAAGTGGGG; primer 14, AACCCTACCCATACGATGTTCCAGATTACGCTTAAATGAATAAAAATAA; primer 15, ATTTAAGCGTAATCTGGAACATCGTATGGGTACGCATAGTCCGGGACGTCATACGGATAGGGTTTAAGTGGGG; primer 16, AACCCTATCCGTATGACGTCCCGGACTATGCGTACCCATACGATGTTCCAGATTACGCT TAAATGAATAAAAATAA; primer 17, ACGGATAAGCGTAATCTGGAACATCGTATGGGTACGCATAGTCCGGGACGTCATACGGATAGGGTTTAAGTGGGG; primer 18, TATGCGTACCCATACGATGTTCCAGATTACGCTTATCCGTATGACGTCCCGGACTATGCGTAAATGAATAAAAATAA; primer 19, ATTTATTCCATCGGCATATATTCTTCTTCTTCGCAGGGTTTAAGTGGGG; primer 20, AACCCTGCGAAGAAGAAGAATATATGCCGATGGAATAAATGAATAAAAATAA; primer 21, ATTTAGGTTTCCGGTTCCGGCGGCGGGGTCGGCGGTTTGGGTTTAAGTGGGG; primer 22, AACCCAAACCGCCGACCCCGCCGCCGGAACCGGAAACCTAAATGAATAAAAATAA.
NA, not applicable.