Fig. 2.

Colocalization of internalized SV40 with EE and LE markers (see also Movie S4 in the supplemental material). (A and B) SV40-AF647 was added to CV-1 cells transfected with Rab7-mRFP and LAMP1-EGFP or Rab9-EYFP and imaged with a spinning-disc confocal microscope at 153 min postwarming. (C) Fluorescently labeled SV40 internalized into CV-1 cells transfected with the constitutively active mutant Rab5Q79L-EGFP and imaged live with a confocal microscope at 160 min postwarming. (D) Fluorescently labeled dextran was added to CV-1 cells for 2 h, incubated overnight, and subjected to SV40-AF647 for binding on ice for 2 h. At 240 min after warming to 37°C, cells were imaged with a confocal microscope.