Table 1.

Thirty hits from the SV40 siRNA infection screen^a

Gene	Description	Protein class	Function	Reduction of infection (%)
RAB5C	RAB5C, member of the RAS oncogene family	Small GTPase	Endosomal protein sorting	−69.9
RAB4A	RAB4A, member of the RAS oncogene family	Small GTPase	Endosomal protein sorting	−68.1
CFL1	Cofilin 1 (nonmuscle)	Actin-binding protein	Cytoskeletal reorganization	−67.5
CBLC	Cas-Br-M (murine) ecotropic retroviral transforming sequence c	E3 ubiquitin protein ligase	Endosomal protein (EGF) sorting	−64.4
PAK2	P21 protein (Cdc42/Rac)-activated kinase 2	Serine/threonine protein kinase	Cytoskeletal reorganization	−63.7
NCK2	NCK adaptor protein 2	Adaptor protein	EGF signaling/cytoskeletal reorganization	−63.3
EHD1	EH domain-containing 1	Adaptor protein	EGF signaling/cytoskeletal reorganization	−63.3
ARHGAP10	Rho GTPase-activating protein 10	GTPase-activating protein	Cytoskeletal reorganization	−62.4
STX5A	Syntaxin 5	SNARE protein	Vesicle fusion/endosome-to-TGN transport	−61.3
RAB5A	RAB5A, member of the RAS oncogene family	Small GTPase	Endosomal protein sorting	−60.8
CBL	Cas-Br-M (murine) ecotropic retroviral transforming sequence	E3 ubiquitin protein ligase	Endosomal protein (EGF) sorting	−60.4
UBASH3A	Ubiquitin-associated and SH3 domain-containing A; TULA	Adaptor protein	Endosomal protein sorting	−58.3
RAB5B	RAB5B, a member of the RAS oncogene family	Small GTPase	Endosomal protein sorting	−56.2
PRKCG	Protein kinase C gamma	Serine/threonine protein kinase	Protein signaling	−55.5
HGS	Hepatocyte growth factor-regulated tyrosine kinase substrate	Adaptor protein	Endosomal protein sorting	−55.5
GRB2	Growth factor receptor-bound protein 2	Adaptor protein	EGF signaling	−55.4
DNM2	Dynamin 2	Small GTPase	Vesicle scission	−55.4
ARRB2	Arrestin beta 2	Adaptor protein	Protein sorting from Golgi complex to endosome	−54.9
NEDD4L	Neural precursor cell expressed, developmentally downregulated 4-like	E3 ubiquitin protein ligase	Endosomal protein (EGF) sorting	−53.9
GGA2	Golgi-associated, gamma-adaptin ear-containing, Arf-binding protein 2	Adaptor protein	Protein sorting/TGN-to-lysosome transport	−53.2
PAK3	P21 protein (Cdc42/Rac)-activated kinase 3	Serine/threonine protein kinase	Cytoskeletal reorganization	−52.9
PIK3C3	Phosphoinositide-3-kinase, class 3	Phosphoinositide-3-kinase	Endosomal protein sorting	−52.8
NEDD4	Neural precursor cell-expressed, developmentally downregulated 4	E3 ubiquitin protein ligase	Endosomal protein (EGF) sorting	−52.2
RAB1A	RAB1A, member of the RAS oncogene family	Small GTPase	Regulator of vesicle traffic from ER to Golgi complex	−51.5
DAB2	Disabled homolog 2, mitogen-responsive phosphoprotein (Drosophila melanogaster)	Adaptor protein	Endosomal protein sorting	−51.3
DYNC2H1	Dynein, cytoplasmic 2, heavy chain 1	Motor protein	Vesicle transport	−51.3
RAB22A	RAB22A, member of the RAS oncogene family	Small GTPase	Endosomal protein sorting	−49.8
PRKCZ	Protein kinase C zeta	Serine/threonine protein kinase	Protein signaling	−49.2
SNX15	Sorting nexin 15	Sorting nexin	Endosomal protein sorting	−48.2
VIL2	Villin-2; ezrin	Actin-binding protein	Cytoskeletal reorganization	−47.6

^aSee also Fig. S1 and S2 in the supplemental material. EGF, epidermal growth factor; TGN, trans-Golgi network; Arf, ADP-ribosylating factor.