Fig. 5.

The SVV IRES directly binds to 40S subunits and eIF3. (A) Binding curves of ³²P-labeled wild-type and IIId₂ deletion mutant RNAs to purified 40S subunits. Labeled RNAs were incubated with 40S subunits and binding assessed by filter binding assay. (B) Binding curves of ³²P-labeled wild-type and IIId₂ deletion mutant RNAs to purified eIF3. Labeled RNAs were incubated with eIF3, and binding was assessed by filter binding assay. Reported values are the average of results from three repetitions with standard errors. All calculations were performed with GraphPad Prism 5.