Fig. 4.
Lipidation of LC3 after infection with virions, dense bodies, or L-particles. (A) HFFF2 monolayers were infected with purified HCMV virions (lanes 1 to 3) or dense bodies (DBs) (lanes 4 to 6) or were mock infected (M) (lane 7). Cell lysates were prepared at 6 hpi for the analysis of LC3 or at 2 hpi for the analysis of pp65 and pp71. The numbers of particles added to 1.5 × 105 cells were 6 × 107 (lanes 1 and 4), 6 × 106 (lanes 2 and 5), or 6 × 105 (lanes 3 and 6). (B) HFFF2 monolayers were mock infected (lane 1) or infected with HSV-1 virions (lanes 2 to 4) or L-particles (lanes 5 to 7), with 100 μg of cycloheximide per milliliter present throughout. Cell lysates were prepared at 6 hpi for the analysis of LC3 or 2 hpi for the analysis of VP16. The number of particles added to 1.5 × 105 cells was 6 × 108 (lanes 2 and 5), 6 × 107 (lanes 3 and 6), or 6 × 106 (lanes 4 and 7).