Figure 5.

Brk represses dpp and zen directly. Transgenic embryos carrying the FLP-out construct eve–stripe 2-brk were hybridized with either brk and dpp (A,C) or brk and zen (B,D) antisense RNA probes. dpp and zen are repressed in the region of eve–stripe 2 (parasegment 3) as Brk protein accumulates during mid- to late cellularization (C,D), but not earlier in precellular stages (A,B). Red arrows delimit the early broad stripe 2 expression. Arrowheads point to the site where normal loss of dpp and zen expression during cellularization occurs.