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. 2001 Feb 1;15(3):328–339. doi: 10.1101/gad.185901

Figure 9.

Figure 9

The WT1 17AA motif confers a survival function in cells exposed to UV light. (A) Embryonic kidney 293 cells were transfected with either empty CMV expression vector or full-length WT1 (−/−), WT1 ( +/−), or WT1 (+/−) G253A under the control of a CMV promoter (4 μg in each case). Sixteen hours after UV light treatment (where indicated), the cells were photographed. (B) Cells were transfected as in A, but along with 1 μg of an expression vector containing green fluorescent protein. Cells were photographed under fluorescence to visualize only the transfected cells. (C) Immunoblot with anti-WT1 antibodies showing that the WT1 +/−, −/−, and +/− G253A derivatives are expressed at equivalent levels in 293 cells. (D) Survival curves to quantify the effect of WT1 +/− and the mutant G253 on cell number after UV light treatment. Each WT1 derivative (0.5, 1, 2, and 4 μg; balanced with empty CMV vector) was transfected into 293 cells, and 16 h after UV light treatment the cells were harvested from the plate and counted. Percentage survival is relative to a control plate of 293 cells transfected with CMV, but not subject to UV light treatment. Each point is the mean average of three independent transfection experiments with standard deviation.