Fig. 7.

MVC attenuates the entry and replication of MVC-Res Env in MDM. (A) Luciferase reporter viruses pseudotyped with MVC-Res or MVC-Sens Envs, or with the nonfunctional ΔKS Env, were used to infect cultures of MDM or PBMC in the presence or absence of 5 μM MVC as described in Materials and Methods. The level of entry is shown by luciferase activity in cell lysates. (B) Replication-competent HIV-1 strains carrying the MVC-Sens or MVC-Res env genes were constructed, titrated, and used to infect MDM cultures in the presence or absence of 1 μM MVC, as described in Materials and Methods. The replication of these viruses and the M-tropic R5 HIV-1 strain AD8 was monitored by measuring reverse transcriptase (RT) activity in culture supernatants. The data shown are means and standard deviations of duplicate (MDM) or triplicate (PBMC) cultures and are representative of 4 independent experiments conducted with cells obtained from different donors.