Fig. 8.

Env-mediated fusion activity influences the replication of MVC-Res Env in MDM. Cell-cell fusion assays using 293T effector cells expressing equivalent levels of JRCSF or YU2 Env (A) or MVC-Sens or MVC-Res Env (B) and MDM as target cells were conducted as described in Materials and Methods. Mock cultures were transfected with the nonfunctional ΔKS Env (A). Fusion levels were scored as described in Materials and Methods. Arrows point to representative fusion events. The experiments were conducted in duplicate, and the results shown are representative of 4 independent experiments conducted with MDM obtained from different donors.