Fig. 4.
Characteristic properties of MAb W226. (A) Biacore analysis of W226 reveals a dissociation constant of 523 × 10−12 M. (B) Immunohistochemical staining of mouse brain tissue. W226 stains PrPSc in tissues of RML-diseased mice. Magnifications, ×10 (upper panels) and ×40 (lower panels). (C) Detection of surface PrP in uninfected Bos2 (top) and ScN2a (bottom) cells by flow cytometry. (D) Detection of intracellular PrP in permeabilized Bos2 (top) and ScN2a (bottom) cells. Flow cytometry was performed using a FACSCalibur flow cytometer (Becton Dickinson [BD], Heidelberg, Germany) with secondary antibodies from BD and a Cytofix/Cytoperm kit (BD) for intracellular staining.