Fig. 3.
Infection of human PBMCs and immortalized Ptm lymphocytes with HIVAQ23/SIVvif carrying the G312V change (a) or the A204E change (b). The p24gag levels are shown as a function of time postinfection in human PBMCs (top graphs) and Ptm lymphocytes (bottom graphs). The data points represent the average measurements from duplicate cultures. The figure key is shown in the top plots, and Adapt. Sup. refers to the uncloned adapted viral quasispecies obtained by long-term culturing from which the mutations were isolated. The results are representative of at least three independent experiments. (c) Infection of primary Ptm PBMCs from two different donors with parental HIVAQ23/SIVvif (light gray diamonds), HIVAQ23/SIVvif G312V (dark gray triangles), or HIVAQ23/SIVvif A204E (black diamonds). The p24gag levels are shown as a function of time postinfection, and data points represent the average measurements from duplicate cultures.