Fig. 4.

CHLA and PUG do not induce IFN-mediated immune response against VSV infection. A549 cells were seeded in 12-well plates and then pretreated with CHLA (60 μM), PUG (40 μM), or IFN-α (1,000 U/ml) or with media and DMSO (0.1%) controls for 24 h. Cell monolayers were washed with PBS twice and then infected with VSV-GFP (MOI = 0.01) for 1 h before overlay media containing 2% FCS and 2% methylcellulose were applied. After an additional 48 h of incubation, the plates were scanned for visualization of fluorescent plaques. The data shown are representative images from one of two independent experiments.