Fig. 6.

CHLA and PUG do not affect HSV-1 transcription, following entry into the host cell. (A) A549 cells were infected with HSV-1 (MOI = 1) for 1 h, treated with low-pH citrate buffer (pH 3.0) to inactivate noninternalized extracellular viral particles, and subsequently overlaid with medium containing CHLA (60 μM), PUG (40 μM), or DMSO control (0.1%). At 4, 8, and 12 h p.i., total cellular RNA was isolated, subjected to first-strand synthesis by reverse transcription, and then amplified by standard PCR procedures with primers against HSV-1 immediate-early (ICP27), early (TK), and late (gD) gene products. GAPDH was included as a control. (B) A549 cells were coincubated with HSV-1 (MOI = 1) and CHLA (60 μM), PUG (40 μM), or DMSO control (0.1%) for 1 h. Cells were washed with PBS before overlay media without the tannins was applied. Total cellular RNA was isolated for RT-PCR analysis as in panel A. Representative data shown are from one of two independent experiments.