Fig. 2.

Properties of LP. (A) E. coli cell growth upon expression of the proposed LP, the individual transmembrane helices 1 and 2 of LP, LP with early termination codon (LP TAA), AP, and the translated sequence of nucleotides 2098 to 2226 in the ϕCb5 genome. All the ORFs were cloned under arabinose-inducible promoter in pBAD plasmid (Invitrogen) and expressed in E. coli strain JM109. Growth of untransformed JM109 is shown as well. (B) Position of LP in the RP gene. Positions of predicted transmembrane regions are shown as “TM1” and “TM2” and actual expressed protein sequences as “helix 1” and “helix 2.” The sequence of the proposed LP, with transmembrane regions in bold italic, is shown.