Table 2.
Assignment of poliovirus-neutralizing Fab clones to their closest human germ line counterparts, based on nucleotide sequence homologya
| Fab | Germ line gene |
||||||
|---|---|---|---|---|---|---|---|
| Heavy chain |
Light chain |
||||||
| V-gene | Identity (%)b | J-gene | D-gene | V-gene | Identity (%) | J-gene | |
| A12 | IGHV4-59*04 | 86 | IGHJ5*02 | IGHD5-12*01 | IGKV1-9*01 | 92 | IGKJ1*01 |
| H2 | IGHV3-30*03 | 88 | IGHJ4*02 | IGHD3-16*02 | IGKV6-21*01 | 96 | IGKJ1*01 |
| A6 | IGHV3-48*01 | 85 | IGHJ4*02 | IGHD4-23*01 | IGKV1-13*02 | 96 | IGLJ4*01 |
| B2 | IGHV3-30*03 | 89 | IGHJ3*01 | IGHD5-12*01 | IGLV1-44*01 | 96 | IGKJ2*01 |
| B10 | IGHV3-7*01 | 90 | IGHJ6*03 | IGHD3-3*01 | IGKV1-9*01 | 90 | IGLJ3*01 |
| C10 | IGHV3-7*01 | 93c | IGHJ6*03 | IGHD3-3*01 | IGKV1-9*01 | 90 | IGLJ3*01 |
a
The closest human V-gene germ lines were identified by searching the IMGT database at http://www.imgt.org/.
b
The VH and VL genes before the CDR3 region were used to calculate the percentage of nucleic acid identity. The mutations in the first 20 bp were excluded since these mutations could be introduced by PCR primers.
c
There was a 3-bp insertion in the CDR2 region in addition to the 93% identity.