Table 3.
Blocking ELISA reaction between human monoclonal antibodies and polioviruses
| MAb | Blocking activity (%) with antigen of typea: |
|||||
|---|---|---|---|---|---|---|
| 1 |
2 |
3 |
||||
| Mean | SD | Mean | SD | Mean | SD | |
| H2 | 0.66 | ± 0.04 | 0 | ± 0.03 | 0 | ± 0.05 |
| A12 | 0.63 | ± 0.06 | 0.73 | ± 0.04 | 0.01 | ± 0.04 |
| A6 | 0.01 | ± 0.04 | 0.97 | ± 0.05 | 0.01 | ± 0.03 |
| B2 | −0.02 | ± 0.05 | 0.97 | ± 0.04 | 0.06 | ± 0.04 |
| B10 | −0.02 | ± 0.03 | 0.03 | ± 0.03 | 0.79 | ± 0.04 |
| C10 | −0.06 | ± 0.04 | −0.06 | ± 0.03 | 0.73 | ± 0.03 |
a
The values represent the relative reductions (blocking) of ELISA reactions between surface-captured antigens and polyclonal conjugates after treatment with monoclonal antibodies and reflect affinities between the antibodies and the antigens. Data in boldface show strong neutralizing interactions.