Fig. 3.

A predicted structural motif in the SARS-CoV E tail is important for Golgi complex targeting. (A) Chimeric G-E mutants contain the N-terminal VSV-G sequence and hydrophobic domain (HD) fused to the C-terminal SARS-CoV E cytoplasmic tail sequence. The amino acid residues predicted to form beta-strands are underlined, alanine replacements within the predicted beta-strands are indicated by the letter “A” in boldface, and the highly conserved proline residue is indicated by the arrow. (B) HeLa cells expressing G-E and mutant G-E proteins (G-E_PA, G-E_Ala4, and G-E_Ala6) were fixed, permeabilized, and labeled with mouse anti-VSV-G.