Fig. 7.

Tim prevents the formation of double-strand breaks at OriP. (A) Mutu I cells (left) or Raji cells (right) were infected with either shControl or shTim, as indicated. Total intact DNA was extracted with agarose plugs digested with BamHI and then assayed by Southern blot analysis with probes for OriP (BamHI C) (A) or OriLyt (detecting OriLyt duplicated sequence in BamHI d and BamHI H) (B). Double-strand breaks are indicated by arrowhead. Molecular mass markers are indicated on the left. A schematic of the BamHI C probe and fragments are shown below panel A. Predicted breakpoints in the OriP region are indicated by the vertical arrow, gel fragments are indicated by horizontal lines, and numbers are derived from the EBV coordinates in GenBank accession no. AJ507799.